Main Article Content
Encephalitis is defined as inflammation of the brain parenchyma associated with neurologic dysfunction. AES occurs in explosive epidemics or in a non-epidemic (sporadic) form. Epidemic in India have been attributed to Japanese encephalitis virus (JEV) infection. Herpes simplex virus (HSV) is responsible for most sporadic cases of AES. This study was undertaken to detect JEV and HSV from CSF samples. Method: Two to three ml of CSF was collected in a dry sterile container. Samples were divided into 2 vials and kept at -20oC. One vial was used for ELISA test in while the other vial was used for PCR. Result: Of 90 cases, 61 were males (68%), 29 were females (32%). Majority belonged to 1 month-5years in 34 (38%) cases followed by 6-10 years in 23 (26%) cases. The commonest symptom was fever in 90 (100%) cases followed by change in mental status in 86 (95.6%).Of 90 samples 2 (2.2%) was positive for JEV in PCR while only 1 (1.1%) was positive in ELISA. A total of 9 (10%) samples were positive for HSV 1 & 2, of which 8 (8.9%) samples were positive in ELISA assay and 8 (8.9%) were positive in PCR assay. In our study only 2 (2.2%) samples were positive for JEV in PCR while one was negative in ELISA. 9 (10%) samples were tested positive for HSV which signifies the sporadic nature of the virus in this region. Conclusion: PCR was found to be more sensitive in detection of JEV, while in HSV detection both ELISA and PCR were equally sensitive.
Key words: Japanese B encephalitis virus; Herpes simplex virus; Acute encephalitis syndrome; Polymerase chain reaction; Enzyme linked immunosorbent assay.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
The submitted papers are assumed to contain no proprietary material unprotected by patent or patent application; responsibility for technical content and for protection of proprietary material rests solely with the author(s) and their organizations and is not the responsibility of the journal. The main (first/corresponding) author is responsible for ensuring that the article has been seen and approved by all the other authors. It is the responsibility of the author to obtain all necessary copyright release permissions for the use of any copyrighted materials in the manuscript prior to the submission.
What are my rights as an author?
It is important to check the policy for the journal to which you are submitting or publishing to establish your rights as
Author. Journal's standard policies allow the following re-use rights:
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to obtain publishing rights without restrictions.
- You may do whatever you wish with the version of the article you submitted to the journal.
- Once the article has been accepted for publication, you may post the accepted version of the article on your own personal website, your department's website or the repository of your institution without any restrictions.
- You may not post the accepted version of the article in any repository other than those listed above (i.e. you may not deposit in the repository of another institution or a subject-matter repository) until 12 months after publication of the article in the journal.
- You may use the published article for your own teaching needs or to supply on an individual basis to research colleagues, provided that such supply is not for commercial purposes.